Coding
L-Ri

Part:BBa_K4383018:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


L-Ri(codon optimisation)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 730
    Illegal NotI site found at 643
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

isomerizing L-erythritol to L-erythrose. Codon optimized according to E. coli. For more information, please view https://2022.igem.wiki/scu-china/design.html.

Source

synthesis according to Acinetobacter sp.

References

Zou X, Lin J, Mao X, Zhao S, Ren Y. Biosynthesis of L-Erythrose by Assembly of Two Key Enzymes in Gluconobacter oxydans. J Agric Food Chem. 2017 Sep 6;65(35):7721-7725. doi: 10.1021/acs.jafc.7b02201. Epub 2017 Aug 24.